产品目录

分子量：Approximately 56.6kDa,a single non-glycosylated polypeptide chain containing 502 amino acids.(MRGSGSHHHHHH-PDI).
储存条件：冷冻（-20℃）

概述

Protein disulfide isomerases(PDIs)constitute a family of structurally related enzymes which catalyze disulfide bonds formation,reduction,or isomerization of newly synthesized proteins in the lumen of the endoplasmic reticulum(ER).They act also as chaperones,and are,therefore,part of a quality-control system for the correct folding of the proteins in the same subcellular compartment.PDI has been found to have moderate effects(25-fold)on the rate of oxidative folding of proteins in vitro.Recombinant Human Protein Disulfide Isomerase is involved in disulphide-bond formation and isomerization,as well as the reduction of disulphide bonds in proteins.Recombinant PDI has been found to have moderate effects(25-fold)on the rate of oxidative folding of proteins in vitro.

使用说明

This material is offered by Biorab Biotech for research,laboratory or further evaluation purposes.NOT FOR HUMAN USE.

技术规格

外观	Sterile Filtered White lyophilized(freeze-dried)powder.
纯度	＞95%by SDS-PAGE and HPLC analyses.
来源	Escherichia coli.
成分	Lyophilized from a 0.2μmfiltered concentrated solution in PBS,pH 7.0.
内毒素	Less than 1EU/μg of rHuPDI as determined by LAL method.
复溶	We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom.Reconstitute in sterile distilled water or aqueous buffer containing 0.1%BSA to a concentration of 0.1-1.0mg/ml.Stock solutions should be apportioned into working aliquots and stored at ≤ -20 ℃.Further dilutions should be made in appropriate buffered solutions.
氨基酸序列	MRGSGSHHHH HHAPEEEDHV LVLRKSNFAE ALAAHKYLLV EFYAPWCGHC KALAPEYAKA AGKLKAEGSE IRLAKVDATE ESDLAQQYGV RGYPTIKFFR NGDTASPKEY TAGREADDIV NWLKKRTGPA ATTLPDGAAA ESLVESSEVA VIGFFKDVES DSAKQFLQAA EAIDDIPFGI TSNSDVFSKY QLDKDGVVLF KKFDEGRNNF EGEVTKENLL DFIKHNQLPL VIEFTEQTAP KIFGGEIKTH ILLFLPKSVS DYDGKLSNFK TAAESFKGKI LFIFIDSDHT DNQRILEFFG LKKEECPAVR LITLEEEMTK YKPESEELTA ERITEFCHRF LEGKIKPHLM SQELPEDWDK QPVKVLVGKN FEDVAFDEKK NVFVEFYAPW CGHCKQLAPI WDKLGETYKD HENIVIAKMD STANEVEAVK VHSFPTLKFF PASADRTVID YNGERTLDGF KKFLESGGQD GAGDDDDLED LEEAEEPDME EDDDQKAVKD EL
Thiol protein Reductase activity	Thiol Protein Reductase Activity is 0.001 Δ650nm/min-2,determined by measuring the turbidity increase at 650 nm due to insulin reduction.The activity is expressed as the ratio of the slope of a linear part of the turbidity curve to the lag time.
Isomerase activity	Isomerase Activity is 0.5 μmol active RNase A min-1 μmol PDI-1,according to the re-activation of reduced and denatured RNase A.

相关搜索：重组人PDI，PDI, Human，Recombinant Human Protein Disulfide Isomerase